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1wae

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Revision as of 08:21, 3 February 2008 by OCA (Talk | contribs)
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1wae, resolution 1.95Å

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CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE

Overview

Copper nitrite reductases contain both an electron-transfer type 1 Cu site, and a catalytic type 2 Cu site. We have mutated one of the type 2 copper, ligating histidines to observe the effect on catalytic turnover. This, mutation has created a unique site where Cu is ligated by 2 His Nepsilon2, atoms alone.

About this Structure

1WAE is a Single protein structure of sequence from Achromobacter xylosoxidans with and as ligands. Active as Transferred entry: 1.7.2.1, with EC number 1.7.99.3 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase., Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS, Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622

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