1wbf
From Proteopedia
|
WINGED BEAN LECTIN, SACCHARIDE FREE FORM
Overview
The crystal structure of the saccharide-free form of the basic form of, winged-bean agglutinin (WBAI) has been solved by the molecular-replacement, method and refined at 2.3 A resolution. The final R factor is 19.7% for, all data in the resolution range 8.0-2.3 A. The asymmetric unit contains, two half-dimers, each located on a crystallographic twofold axis. The, structure of the saccharide-free form is compared with that of the complex, of WBAI with methyl-alpha-D-galactoside. The complex is composed of two, dimers in the asymmetric unit. The intersubunit interactions in the dimer, are nearly identical in the two structures. The binding site of the, saccharide-free structure contains three ordered water molecules at, positions similar to those of the hydroxyl groups of the carbohydrate, which are hydrogen bonded to the protein. Superposition of the, saccharide-binding sites of the two structures shows that the major, changes involve expulsion of these ordered water molecules and a shift of, about 0.6 A of the main-chain atoms of the variable loop.
About this Structure
1WBF is a Single protein structure of sequence from Psophocarpus tetragonolobus with NAG, MN and CA as ligands. Full crystallographic information is available from OCA.
Reference
Structure of basic winged-bean lectin and a comparison with its saccharide-bound form., Manoj N, Srinivas VR, Suguna K, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310
Page seeded by OCA on Wed Nov 21 05:17:36 2007
