1wki

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1wki Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

solution structure of ribosomal protein L16 from thermus thermophilus HB8

Overview

Ribosomal protein L16 is an essential component of the bacterial ribosome., It organizes the architecture of aminoacyl tRNA binding site in the, ribosome 50S subunit. The three-dimensional structure of L16 from Thermus, thermophilus HB8 was determined by NMR. In solution, L16 forms an, alpha+beta sandwich structure combined with two additional beta sheets, located at the loop regions connecting the two layers. The terminal, regions and a central loop region did not show any specific secondary, structure. The structured part of L16 could be superimposed well on the, C(alpha) model of L16 determined in the crystal structure of the ribosome, 50S subunit. By overlaying the L16 solution structure onto the coordinates, of the ribosome crystal structure, we constructed the combined model that, represents the ribosome-bound state of L16 in the detailed structure. The, model showed that L16 possesses residues in contact with helices 38, 39, 42, 43 and 89 of 23S rRNA and helix 4 of 5S rRNA. This suggests its broad, effect on the ribosome architecture. Comparison of L16 with the L10e, protein, which is the archaeal counterpart, showed that they share a, common fold, but differ in some regions of functional importance, especially in the N-terminal region. All known mutation sites in L16 that, confer resistance to avilamycin and evernimicin were positioned so that, their side-chains were exposed to solvent in the internal cavity of the, ribosome. This suggests the direct participation of L16 as a part of the, binding site for antibiotics.

About this Structure

1WKI is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Solution structure of ribosomal protein L16 from Thermus thermophilus HB8., Nishimura M, Yoshida T, Shirouzu M, Terada T, Kuramitsu S, Yokoyama S, Ohkubo T, Kobayashi Y, J Mol Biol. 2004 Dec 10;344(5):1369-83. PMID:15561149

Page seeded by OCA on Wed Nov 21 05:29:24 2007