1wlf

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spinqualitylabelsall models 1wlf, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structure of the N-terminal domain of PEX1 AAA-ATPase: Characterization of a putative adaptor-binding domain

Overview

Peroxisomes are responsible for several pathways in primary metabolism, including beta-oxidation and lipid biosynthesis. PEX1 and PEX6 are, hexameric AAA-type ATPases, both of which are indispensable in targeting, over 50 peroxisomal resident proteins from the cytosol to the peroxisomes., Although the tandem AAA-ATPase domains in the central region of PEX1 and, PEX6 are highly similar, the N-terminal sequences are unique. To better, understand the distinct molecular function of these two proteins, we, analyzed the unique N-terminal domain (NTD) of PEX1. Extensive, computational analysis revealed weak similarity (<10% identity) of PEX1, NTD to the N-terminal domains of other membrane-related type II, AAA-ATPases, such as VCP (p97) and NSF. We have determined the crystal, structure of mouse PEX1 NTD at 2.05-A resolution, which clearly, demonstrated that the domain belongs to the double-psi-barrel fold family, found in the other AAA-ATPases. The N-domains of both VCP and NSF are, structural neighbors of PEX1 NTD with a 2.7- and 2.1-A root mean square, deviation of backbone atoms, respectively. Our findings suggest that the, supradomain architecture, which is composed of a single N-terminal domain, followed by tandem AAA domains, is a common feature of organellar, membrane-associating AAA-ATPases. We propose that PEX1 functions as a, protein unfoldase in peroxisomal biogenesis, using its N-terminal putative, adaptor-binding domain.

About this Structure

1WLF is a Protein complex structure of sequences from Mus musculus with SO4 as ligand. Full crystallographic information is available from OCA.

Reference

Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain., Shiozawa K, Maita N, Tomii K, Seto A, Goda N, Akiyama Y, Shimizu T, Shirakawa M, Hiroaki H, J Biol Chem. 2004 Nov 26;279(48):50060-8. Epub 2004 Aug 24. PMID:15328346

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