1wyu

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spinqualitylabelsall models 1wyu, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of glycine decarboxylase (P-protein) of the glycine cleavage system, in holo form

Overview

The crystal structure of the P-protein of the glycine cleavage system from, Thermus thermophilus HB8 has been determined. This is the first reported, crystal structure of a P-protein, and it reveals that P-proteins do not, involve the alpha(2)-type active dimer universally observed in the, evolutionarily related pyridoxal 5'-phosphate (PLP)-dependent enzymes., Instead, novel alphabeta-type dimers associate to form an alpha(2)beta(2), tetramer, where the alpha- and beta-subunits are structurally similar and, appear to have arisen by gene duplication and subsequent divergence with a, loss of one active site. The binding of PLP to the apoenzyme induces large, open-closed conformational changes, with residues moving up to 13.5 A. The, structure of the complex formed by the holoenzyme bound to an inhibitor, (aminooxy)acetate, suggests residues that may be responsible for substrate, recognition. The molecular surface around the lipoamide-binding channel, shows conservation of positively charged residues, which are possibly, involved in complex formation with the H-protein. These results provide, insights into the molecular basis of nonketotic hyperglycinemia.

About this Structure

1WYU is a Protein complex structure of sequences from Thermus thermophilus with PLP as ligand. Active as Glycine dehydrogenase (decarboxylating), with EC number 1.4.4.2 Full crystallographic information is available from OCA.

Reference

Structure of P-protein of the glycine cleavage system: implications for nonketotic hyperglycinemia., Nakai T, Nakagawa N, Maoka N, Masui R, Kuramitsu S, Kamiya N, EMBO J. 2005 Apr 20;24(8):1523-36. Epub 2005 Mar 24. PMID:15791207

Page seeded by OCA on Sun Nov 25 01:04:23 2007