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1h30

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Revision as of 10:25, 30 October 2007 by OCA (Talk | contribs)
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1h30, resolution 2.2Å

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C-TERMINAL LG DOMAIN PAIR OF HUMAN GAS6

Overview

Receptor tyrosine kinases of the Axl family are activated by Gas6, the, product of growth arrest-specific gene 6. Gas6-Axl signaling is implicated, in cell survival, adhesion, and migration. The receptor-binding site of, Gas6 is located within a C-terminal pair of laminin G-like (LG) domains, that do not resemble any other receptor tyrosine kinase ligand. We report, the crystal structure at 2.2-A resolution of a Gas6 fragment spanning both, LG domains (Gas6-LG). The structure reveals a V-shaped arrangement of LG, domains strengthened by an interdomain calcium-binding site. LG2 of, Gas6-LG contains two unusual features: an alpha-helix cradled by one edge, of the LG beta-sandwich and a conspicuous patch of surface-exposed, hydrophobic residues. Mutagenesis of some residues in this patch ... [(full description)]

About this Structure

1H30 is a [Single protein] structure of sequence from [Homo sapiens] with CA and SO4 as [ligands]. Structure known Active Site: CAA. Full crystallographic information is available from [OCA].

Reference

Crystal structure of a C-terminal fragment of growth arrest-specific protein Gas6. Receptor tyrosine kinase activation by laminin G-like domains., Sasaki T, Knyazev PG, Cheburkin Y, Gohring W, Tisi D, Ullrich A, Timpl R, Hohenester E, J Biol Chem. 2002 Nov 15;277(46):44164-70. Epub 2002 Sep 5. PMID:12218057

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