1xsh
From Proteopedia
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Solution structure of E.coli RNase P RNA P4 stem oligoribonucleotide, U69C/C70U mutation
Overview
The solution structures of two 27 nt RNA hairpins and their complexes with, cobalt(III)-hexammine [Co(NH(3))(6)(3+)] were determined by NMR, spectroscopy. The RNA hairpins are variants of the P4 region from, Escherichia coli RNase P RNA: a U-to-A mutant changing the identity of the, bulged nucleotide, and a U-to-C, C-to-U double mutant changing only the, bulge position. Structures calculated from NMR constraints show that the, RNA hairpins adopt different conformations. In the U-to-C, C-to-U double, mutant, the conserved bulged uridine in the P4 wild-type stem is found to, be shifted in the 3'-direction by one nucleotide when compared with the, wild-type structure. Co(NH(3))(6)(3+) is used as a spectroscopic probe for, Mg(H(2)O)(6)(2+) binding sites because both complexes have octahedral, symmetry and have similar radii. Intermolecular NOE crosspeaks between, Co(NH(3))(6)(3+) and RNA protons were used to locate the site of, Co(NH(3))(6)(3+) binding to both RNA hairpins. The metal ion binds in the, major groove near a bulge loop in both mutants, but is shifted 3' by about, one base pair in the double mutant. The change of the metal ion binding, site is compared with results obtained on corresponding mutant RNase P RNA, molecules as reported by Harris and co-workers (RNA, 1, 210-218).
About this Structure
1XSH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Change of RNase P RNA function by single base mutation correlates with perturbation of metal ion binding in P4 as determined by NMR spectroscopy., Schmitz M, Nucleic Acids Res. 2004 Dec 2;32(21):6358-66. Print 2004. PMID:15576680
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