1xtb

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spinqualitylabelsall models 1xtb, resolution 2.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of Rabbit Phosphoglucose Isomerase Complexed with Sorbitol-6-Phosphate

Overview

Phosphoglucose isomerase (PGI) catalyzes the isomerization of, D-glucose-6-phosphate (G6P) and D-fructose-6-phosphate (F6P) in glycolysis, and gluconeogenesis. Analysis of previously reported X-ray crystal, structures of PGI without ligand, with the cyclic form of F6P, or with, inhibitors that mimic the cis-enediol intermediate led to proposed, mechanisms for the ring opening and isomerization steps in the multistep, catalytic mechanism. To help complete our model of the overall mechanism, information is needed about the state of PGI between the ring opening and, isomerization steps, in other words, a structure of the enzyme complexed, with the open form of a substrate or an analog. Here, we report the, crystal structure of rabbit PGI complexed with D-sorbitol-6-phosphate, (S6P), an analog of the open chain form of G6P, at 2.0 A resolution. As, was seen in the PGI/F6P structure, a helix containing amino acid residues, 512-520 is found in the "out" position, which provides sufficient space in, the active site for a substrate in its cyclic form and which is probably, the location of that helix just after ring opening (or just before ring, closure). However, the S6P ligand is in an extended conformation, as was, seen previously with ligands that mimic the cis-enediol intermediate. The, extended conformation enables the ligand to interact with Glu357, which, transfers a proton during the isomerization step. The PGI/S6P structure, represents the conformation of the enzyme and substrate between the ring, opening (or ring closing) step and the isomerization step and helps to, complete the model for PGI's catalytic mechanism.

About this Structure

1XTB is a Single protein structure of sequence from Oryctolagus cuniculus with S6P as ligand. Active as Glucose-6-phosphate isomerase, with EC number 5.3.1.9 Full crystallographic information is available from OCA.

Reference

The crystal structure of rabbit phosphoglucose isomerase complexed with D-sorbitol-6-phosphate, an analog of the open chain form of D-glucose-6-phosphate., Lee JH, Jeffery CJ, Protein Sci. 2005 Mar;14(3):727-34. Epub 2005 Feb 2. PMID:15689508

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