1y9t
From Proteopedia
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Crystal structure of a type III secretion system protein complexed with the lipid, 1-monohexanoyl-2-hydroxy-sn-glycero-3-phosphate
Overview
The ability to translocate virulence proteins into host cells through a, type III secretion apparatus (TTSS) is a hallmark of several Gram-negative, pathogens including Shigella, Salmonella, Yersinia, Pseudomonas, and, enteropathogenic Escherichia coli. In common with other types of bacterial, secretion apparatus, the assembly of the TTSS complex requires the, preceding formation of its integral outer membrane secretin ring, component. We have determined at 1.5 A the structure of MxiM28-142, the, Shigella pilot protein that is essential for the assembly and membrane, association of the Shigella secretin, MxiD. This represents the first, atomic structure of a secretin pilot protein from the several bacterial, secretion systems containing an orthologous secretin component. A deep, hydrophobic cavity is observed in the novel 'cracked barrel' structure of, MxiM, providing a specific binding domain for the acyl chains of bacterial, lipids, a proposal that is supported by our various lipid/MxiM complex, structures. Isothermal titration analysis shows that the C-terminal domain, of the secretin, MxiD525-570, hinders lipid binding to MxiM.
About this Structure
1Y9T is a Single protein structure of sequence from Shigella flexneri with ACT and HHG as ligands. Full crystallographic information is available from OCA.
Reference
Structure and biochemical analysis of a secretin pilot protein., Lario PI, Pfuetzner RA, Frey EA, Creagh L, Haynes C, Maurelli AT, Strynadka NC, EMBO J. 2005 Mar 23;24(6):1111-21. Epub 2005 Mar 10. PMID:15775974
Page seeded by OCA on Wed Nov 21 06:38:40 2007
