1ynk
From Proteopedia
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Identification of Key residues of the NC6.8 Fab antibody fragment binding to synthetic sweeteners: Crystal structure of NC6.8 co-crystalized with high potency sweetener compound SC45647
Overview
The crystal structures of the murine monoclonal IgG2b(kappa) antibody, NC6.8 Fab fragment complexed with high-potency sweetener compound SC45647, and nontasting high-affinity antagonist TES have been determined. The, crystal structures show how sweetener potency is fine-tuned by multiple, interactions between specific receptor residues and the functionally, different groups of the sweeteners. Comparative analysis with the, structure of NC6.8 complexed with the super-potency sweetener NC174, reveals that although the same residues in the antigen binding pocket of, NC6.8 interact with the zwitterionic, trisubstituted guanidinium, sweeteners as well as TES, specific differences exist and provide guidance, for the design of new artificial sweeteners. In case of the nonsweetener, TES, the interactions with the receptor are indirectly mediated through a, hydrogen bonded water network, while the sweeteners bind with high, affinity directly to the receptor. The presence of a hydrophobic group, interacting with multiple receptor residues as a major determinant for, sweet taste has been confirmed. The nature of the hydrophobic group is, likely a discriminator for super- versus high-potency sweeteners, which, can be exploited in the design of new, highly potent sweetener compounds., Overall similarities and partial conservation of interactions indicate, that the NC6.8 Fab surrogate is representing crucial features of the T1R2, taste receptor VFTM binding site.
About this Structure
1YNK is a Protein complex structure of sequences from Mus musculus with SC5 as ligand. Full crystallographic information is available from OCA.
Reference
Cocrystal structures of NC6.8 Fab identify key interactions for high potency sweetener recognition: implications for the design of synthetic sweeteners., Gokulan K, Khare S, Ronning DR, Linthicum SD, Sacchettini JC, Rupp B, Biochemistry. 2005 Jul 26;44(29):9889-98. PMID:16026161
Page seeded by OCA on Sun Nov 18 09:45:32 2007
