1yq4
From Proteopedia
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Avian respiratory complex ii with 3-nitropropionate and ubiquinone
Overview
We report three new structures of mitochondrial respiratory Complex II, (succinate ubiquinone oxidoreductase, E.C. 1.3.5.1) at up to 2.1 A, resolution, with various inhibitors. The structures define the, conformation of the bound inhibitors and suggest the residues involved in, substrate binding and catalysis at the dicarboxylate site. In particular, they support the role of Arg(297) as a general base catalyst accepting a, proton in the dehydrogenation of succinate. The dicarboxylate ligand in, oxaloacetate-containing crystals appears to be the same as that reported, for Shewanella flavocytochrome c treated with fumarate. The plant and, fungal toxin 3-nitropropionic acid, an irreversible inactivator of, succinate dehydrogenase, forms a covalent adduct with the side chain of, Arg(297). The modification eliminates a trypsin cleavage site in the, flavoprotein, and tandem mass spectroscopic analysis of the new fragment, shows the mass of Arg(297) to be increased by 83 Da and to have the, potential of losing 44 Da, consistent with decarboxylation, during, fragmentation.
About this Structure
1YQ4 is a Protein complex structure of sequences from Gallus gallus with BHG, FAD, FES, SF4, F3S, HEM, 3NP, UQ, PEE and GOL as ligands. Active as Succinate dehydrogenase (ubiquinone), with EC number 1.3.5.1 Full crystallographic information is available from OCA.
Reference
3-nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme., Huang LS, Sun G, Cobessi D, Wang AC, Shen JT, Tung EY, Anderson VE, Berry EA, J Biol Chem. 2006 Mar 3;281(9):5965-72. Epub 2005 Dec 21. PMID:16371358
Page seeded by OCA on Sun Nov 25 04:29:12 2007
Categories: Gallus gallus | Protein complex | Succinate dehydrogenase (ubiquinone) | Anderson, V.E. | Berry, E.A. | Cobessi, D. | Huang, L. | Shen, J.T. | Sun, G. | Tung, E.Y. | Wang, A. | 3NP | BHG | F3S | FAD | FES | GOL | HEM | PEE | SF4 | UQ | Complex ii | Cytochrome b | Heme protein | Iron sulfur protein | Membrane protein | Oxaloacetate nitropropionate ubiquinone | Oxidoreductase | Redox enzyme | Respiratory chain
