1ysd
From Proteopedia
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Yeast Cytosine Deaminase Double Mutant
Overview
Thermostabilizing an enzyme while maintaining its activity for industrial, or biomedical applications can be difficult with traditional selection, methods. We describe a rapid computational approach that identified three, mutations within a model enzyme that produced a 10 degrees C increase in, apparent melting temperature T(m) and a 30-fold increase in half-life at, 50 degrees C, with no reduction in catalytic efficiency. The effects of, the mutations were synergistic, giving an increase in excess of the sum of, their individual effects. The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required, its activity, thereby coupling molecular and metabolic engineering.
About this Structure
1YSD is a Single protein structure of sequence from Saccharomyces cerevisiae with ZN and CA as ligands. Active as Cytosine deaminase, with EC number 3.5.4.1 Full crystallographic information is available from OCA.
Reference
Computational thermostabilization of an enzyme., Korkegian A, Black ME, Baker D, Stoddard BL, Science. 2005 May 6;308(5723):857-60. PMID:15879217
Page seeded by OCA on Wed Nov 21 07:01:18 2007
