1yuj
From Proteopedia
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SOLUTION NMR STRUCTURE OF THE GAGA FACTOR/DNA COMPLEX, 50 STRUCTURES
Overview
The structure of a complex between the DNA binding domain of the GAGA, factor (GAGA-DBD) and an oligonucleotide containing its GAGAG consensus, binding site has been determined by nuclear magnetic resonance, spectroscopy. The GAGA-DBD comprises a single classical Cys2-His2 zinc, finger core, and an N-terminal extension containing two highly basic, regions, BR1 and BR2. The zinc finger core binds in the major groove and, recognizes the first three GAG bases of the consensus in a manner similar, to that seen in other classical zinc finger-DNA complexes. Unlike the, latter, which require tandem zinc finger repeats with a minimum of two, units for high affinity binding, the GAGA-DBD makes use of only a single, finger complemented by BR1 and BR2. BR2 forms a helix that interacts in, the major groove recognizing the last G of the consensus, while BR1 wraps, around the DNA in the minor groove and recognizes the A in the fourth, position of the consensus. The implications of the structure of the, GAGA-DBD-DNA complex for chromatin remodelling are discussed.
About this Structure
1YUJ is a Single protein structure of sequence from Drosophila melanogaster with ZN as ligand. Full crystallographic information is available from OCA.
Reference
The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode., Omichinski JG, Pedone PV, Felsenfeld G, Gronenborn AM, Clore GM, Nat Struct Biol. 1997 Feb;4(2):122-32. PMID:9033593
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