1z1c

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spinqualitylabelsall models 1z1c, resolution 3.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute virus of Mice

Overview

Two strains of the parvovirus minute virus of mice (MVM), the, immunosuppressive (MVMi) and the prototype (MVMp) strains, display, disparate in vitro tropism and in vivo pathogenicity. We report the, crystal structures of MVMp virus-like particles (MVMp(b)) and native, wild-type (wt) empty capsids (MVMp(e)), determined and refined to 3.25 and, 3.75 A resolution, respectively, and their comparison to the structure of, MVMi, also refined to 3.5 A resolution in this study. A comparison of the, MVMp(b) and MVMp(e) capsids showed their structures to be the same, providing structural verification that some heterologously expressed, parvovirus capsids are indistinguishable from wt capsids produced in host, cells. The structures of MVMi and MVMp capsids were almost identical, but, local surface conformational differences clustered from symmetry-related, capsid proteins at three specific domains: (i) the icosahedral fivefold, axis, (ii) the "shoulder" of the protrusion at the icosahedral threefold, axis, and (iii) the area surrounding the depression at the icosahedral, twofold axis. The latter two domains contain important determinants of MVM, in vitro tropism (residues 317 and 321) and forward mutation residues, (residues 399, 460, 553, and 558) conferring fibrotropism on MVMi., Furthermore, these structural differences between the MVM strains, colocalize with tropism and pathogenicity determinants mapped for other, autonomous parvovirus capsids, highlighting the importance of common, parvovirus capsid regions in the control of virus-host interactions.

About this Structure

1Z1C is a Single protein structure of sequence from Minute virus of mice with CA and D5M as ligands. Full crystallographic information is available from OCA.

Reference

Structural determinants of tissue tropism and in vivo pathogenicity for the parvovirus minute virus of mice., Kontou M, Govindasamy L, Nam HJ, Bryant N, Llamas-Saiz AL, Foces-Foces C, Hernando E, Rubio MP, McKenna R, Almendral JM, Agbandje-McKenna M, J Virol. 2005 Sep;79(17):10931-43. PMID:16103145

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