1zc5
From Proteopedia
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Structure of the RNA signal essential for translational frameshifting in HIV-1
Overview
Many pathogenic viruses use a programmed -1 translational frameshifting, mechanism to regulate synthesis of their structural and enzymatic, proteins. Frameshifting is vital for viral replication. A slippery, sequence bound at the ribosomal A and P sites as well as a downstream, stimulatory RNA structure are essential for frameshifting. Conflicting, data have been reported concerning the structure of the downstream RNA, signal in human immunodeficiency virus type 1 (HIV-1). Here, the solution, structure of the HIV-1 frameshifting RNA signal was solved by, heteronuclear NMR spectroscopy. This structure reveals a long hairpin fold, with an internal three-nucleotide bulge. The internal loop introduces a, bend between the lower and upper helical regions, a structural feature, often seen in frameshifting pseudoknots. The NMR structure correlates with, chemical probing data. The upper stem rich in conserved G-C Watson-Crick, base-pairs is highly stable, whereas the bulge region and the lower stem, are more flexible.
About this Structure
1ZC5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the RNA signal essential for translational frameshifting in HIV-1., Gaudin C, Mazauric MH, Traikia M, Guittet E, Yoshizawa S, Fourmy D, J Mol Biol. 2005 Jun 24;349(5):1024-35. PMID:15907937
Page seeded by OCA on Thu Nov 8 14:38:24 2007
