1zjn

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spinqualitylabelsall models 1zjn, resolution 2.61Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus with dGTP

Overview

DNA polymerases occasionally insert the wrong nucleotide. For this error, to become a mutation, the mispair must be extended. We report a structure, of DNA polymerase beta (pol beta) with a DNA mismatch at the boundary of, the polymerase active site. The structure of this complex indicates that, the templating adenine of the mispair stacks with the primer terminus, adenine while the templating (coding) cytosine is flipped out of the DNA, helix. Soaking the crystals of the binary complex with dGTP resulted in, crystals of a ternary substrate complex. In this case, the templating, cytosine is observed within the DNA helix and forms Watson-Crick hydrogen, bonds with the incoming dGTP. The adenine at the primer terminus has, rotated into a syn-conformation to interact with the opposite adenine in a, planar configuration. Yet, the 3'-hydroxyl on the primer terminus is out, of position for efficient nucleotide insertion.

About this Structure

1ZJN is a Single protein structure of sequence from Homo sapiens with MG, NA and DGT as ligands. Full crystallographic information is available from OCA.

Reference

Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate., Batra VK, Beard WA, Shock DD, Pedersen LC, Wilson SH, Structure. 2005 Aug;13(8):1225-33. PMID:16084394

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