232d
From Proteopedia
|
THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)
Overview
The crystal structure of the DNA decamer d(AGGCATGCCT) has been determined, to a resolution of 1.3 A and R factor of 13.9%. The structure has a unique, conformation with each of the decamer single strands forming base-pairing, interactions with two symmetry-related strands. The central eight bases of, the decamer form an A-DNA octamer duplex with one symmetry-related strand, whilst the terminal 5'-A and T-3' bases are flipped out and away from the, octamer helix axis to form base-pairing interactions with a second, symmetry-related strand. These A.T base-pairs lie perpendicular to the, crystallographic c axis and pack within the unit cell in conjunction with, a symmetry-related A.T base-pair displaced by 3.4 A degrees along the c, axis. A novel base triplet interaction of the type A*(G.C) is present in, the structure with interaction from the major groove side of the terminal, 5'-A base to the minor groove of the central A-DNA octamer. This structure, reports the first example of cobalt hexammine binding to a right-handed, DNA duplex. The crystallographic asymmetric unit contains two cobalt, hexammine ligands with one site in the major groove coordinating via, hydrogen bonds to the 5'-AGG bases, and the second site located between, DNA molecules and interacting with the oxygen atoms of phosphate groups.
About this Structure
232D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The high resolution crystal structure of the DNA decamer d(AGGCATGCCT)., Nunn CM, Neidle S, J Mol Biol. 1996 Feb 23;256(2):340-51. PMID:8594201
Page seeded by OCA on Tue Jan 29 17:48:15 2008
