289d
From Proteopedia
|
TARGETING THE MINOR GROOVE OF DNA: CRYSTAL STRUCTURES OF TWO COMPLEXES BETWEEN FURAN DERIVATIVES OF BERENIL AND THE DNA DODECAMER D(CGCGAATTCGCG)2
Overview
Crystal structures are reported of complexes of two novel furan, derivatives of berenil with alkyl benzamidine groups bound to the DNA, sequence d(CGCGAATTCGCG)2. They have both been determined to 2.2 A, resolution and refined to R factors of 16.9% and 18.6%. In both structures, the alkyl substituents, cyclopropyl and isopropyl, are found to be, orientated away from the floor of the minor groove. The drugs are located, in the minor groove by two strong amidinium hydrogen bonds, to the O2 of, the thymines situated at the 5' and 3' ends of the AT-rich region. The, isopropyl-substituted derivative has a tight hydrogen-bonded water network, in the minor groove at one amidine site, which alters the orientation of, the isopropyl substituent. This compound has superior DNA-binding, properties and activity against Pneumocystis carinii and Cryptosporidium, parvum infections in vivo compared to the cyclopropyl derivative, which in, turn is superior to the parent furan compound. We suggest that the nature, and extent of the interactions of these compounds in the DNA minor groove, play an important role in these activities, possibly in conjunction with a, DNA-binding protein. The overall effect of these alkyl benzamidine, substitutions is to increase the binding of the drugs to the minor groove.
About this Structure
289D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Targeting the minor groove of DNA: crystal structures of two complexes between furan derivatives of berenil and the DNA dodecamer d(CGCGAATTCGCG)2., Trent JO, Clark GR, Kumar A, Wilson WD, Boykin DW, Hall JE, Tidwell RR, Blagburn BL, Neidle S, J Med Chem. 1996 Nov 8;39(23):4554-62. PMID:8917643
Page seeded by OCA on Tue Jan 29 17:50:51 2008
