2ab0
From Proteopedia
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Crystal Structure of E. coli protein YajL (ThiJ)
Overview
The Escherichia coli protein YajL (ThiJ) is a member of the DJ-1, superfamily with close homologues in many prokaryotes. YajL also shares, 40% sequence identity with human DJ-1, an oncogene and neuroprotective, protein whose loss-of-function mutants are associated with certain types, of familial, autosomal recessive Parkinsonism. We report the 1.1 angstroms, resolution crystal structure of YajL in a crystal form with two molecules, in the asymmetric unit. The structure of YajL is remarkably similar to, that of human DJ-1 (0.9 angstroms C(alpha) RMSD) and both proteins adopt, the same dimeric structure. The conserved cysteine residue located in the, "nucleophile elbow" is oxidized to either cysteine sulfenic or sulfinic, acid in the two molecules in the asymmetric unit, and a mechanism for this, oxidation is proposed that may be valid for other proteins in the DJ-1, superfamily as well. Rosenfield difference matrix analysis of the refined, anisotropic displacement parameters in the YajL structure reveals, significant differences in the intramolecular flexibility of the two, non-crystallographic symmetry-related molecules in the asymmetric unit., Lastly, a comparison of the crystal structures of the four different, E.coli members of the DJ-1 superfamily indicates that the variable, oligomerization in this superfamily is due to a combination of, protein-specific insertions into the core fold that form specific, interfaces while occluding others plus optimization of residues in the, structurally invariant regions of the core fold that facilitate, protein-protein interactions.
About this Structure
2AB0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The atomic resolution crystal structure of the YajL (ThiJ) protein from Escherichia coli: a close prokaryotic homologue of the Parkinsonism-associated protein DJ-1., Wilson MA, Ringe D, Petsko GA, J Mol Biol. 2005 Oct 28;353(3):678-91. Epub 2005 Sep 2. PMID:16181642
Page seeded by OCA on Wed Nov 21 08:01:47 2007
