2acf

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spinqualitylabelsall models 2acf, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of ADRP domain of SARS NSP3

Overview

The crystal structure of a conserved domain of nonstructural protein 3, (nsP3) from severe acute respiratory syndrome coronavirus (SARS-CoV) has, been solved by single-wavelength anomalous dispersion to 1.4 A resolution., The structure of this "X" domain, seen in many single-stranded RNA, viruses, reveals a three-layered alpha/beta/alpha core with a, macro-H2A-like fold. The putative active site is a solvent-exposed cleft, that is conserved in its three structural homologs, yeast Ymx7, Archeoglobus fulgidus AF1521, and Er58 from E. coli. Its sequence is, similar to yeast YBR022W (also known as Poa1P), a known phosphatase that, acts on ADP-ribose-1-phosphate (Appr-1-p). The SARS nsP3 domain, readily removes the 1 phosphate group from Appr-1-p in in vitro, assays, confirming its phosphatase activity. Sequence and structure, comparison of all known macro-H2A domains combined with available, functional data suggests that proteins of this superfamily form an, emerging group of nucleotide phosphatases that dephosphorylate Appr-1-p.

About this Structure

2ACF is a Single protein structure of sequence from Sars coronavirus with GOL as ligand. Full crystallographic information is available from OCA.

Reference

Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1-phosphate dephosphorylation by a conserved domain of nsP3., Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K, Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P, Structure. 2005 Nov;13(11):1665-75. PMID:16271890

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