2afp
From Proteopedia
|
THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY
Overview
A recombinant form of the sea raven type II antifreeze protein (SRAFP) has, been produced using the Pichia pastoris expression system. The antifreeze, activity of recombinant SRAFP is indistinguishable from that of the, wild-type protein. The global fold of SRAFP has been determined by, two-dimensional 1H homonuclear and three-dimensional 1H- inverted question, mark15N inverted question mark heteronuclear NMR spectroscopy using 785, NOE distance restraints and 47 angular restraints. The molecule folds into, one globular domain that consists of two helices and nine beta-strands in, two beta-sheets. The structure confirms the proposed existence of five, disulfide bonds. The global fold of SRAFP is homologous to C-type lectins, and pancreatic stone proteins, even though the sequence identity is only, approximately 20%.
About this Structure
2AFP is a Single protein structure of sequence from Hemitripterus americanus. Full crystallographic information is available from OCA.
Reference
The solution structure of type II antifreeze protein reveals a new member of the lectin family., Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD, Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:9537986
Page seeded by OCA on Wed Nov 21 08:06:00 2007
