2az1

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Image:2az1.gif

2az1, resolution 2.35Å

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Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum

Overview

Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium, salinarum was crystallized in a free state and a substrate-bound form with, CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged, enzyme, whereas the untagged form was used for co-crystallization with the, nucleotide. Crosslinking under different salt and pH conditions revealed a, stronger oligomerization tendency for the tagged protein at low and high, salt concentrations. The influence of the His6-tag on the halophilic, nature of the enzyme is discussed on the basis of the observed structural, properties.

About this Structure

2AZ1 is a Single protein structure of sequence from Halobacterium salinarum with CA as ligand. Active as Nucleoside-diphosphate kinase, with EC number 2.7.4.6 Full crystallographic information is available from OCA.

Reference

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum., Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D, FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. PMID:16293253

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