2bw3
From Proteopedia
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THREE-DIMENSIONAL STRUCTURE OF THE HERMES DNA TRANSPOSASE
Overview
Mobile elements and their inactive remnants account for large proportions, of most eukaryotic genomes, where they have had central roles in genome, evolution. Over 50 years ago, McClintock reported a form of stress-induced, genome instability in maize in which discrete DNA segments move between, chromosomal locations. Our current mechanistic understanding of enzymes, catalyzing transposition is largely limited to prokaryotic transposases., The Hermes transposon from the housefly is part of the eukaryotic hAT, superfamily that includes hobo from Drosophila, McClintock's maize, Activator and Tam3 from snapdragon. We report here the three-dimensional, structure of a functionally active form of the transposase from Hermes at, 2.1-A resolution. The Hermes protein has some structural features of, prokaryotic transposases, including a domain with a retroviral integrase, fold. However, this domain is disrupted by the insertion of an additional, domain. Finally, transposition is observed only when Hermes assembles into, a hexamer.
About this Structure
2BW3 is a Protein complex structure of sequences from Musca domestica. Full crystallographic information is available from OCA.
Reference
Molecular architecture of a eukaryotic DNA transposase., Hickman AB, Perez ZN, Zhou L, Musingarimi P, Ghirlando R, Hinshaw JE, Craig NL, Dyda F, Nat Struct Mol Biol. 2005 Aug;12(8):715-21. Epub 2005 Jul 24. PMID:16041385
Page seeded by OCA on Tue Jan 29 18:31:43 2008
