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2c2i

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Revision as of 06:52, 21 November 2007 by OCA (Talk | contribs)
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Image:2c2i.jpg

2c2i, resolution 1.80Å

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STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS

Overview

A large fraction of the Mycobacterium tuberculosis genome codes for, proteins of unknown function. We here report the structure of one of these, proteins, Rv0130, solved to a resolution of 1.8 a. The Rv0130 monomer, features a single hotdog fold composed of a highly curved beta-sheet on, top of a long and a short alpha-helix. Two monomers in turn pack to form a, double-hotdog-folded homodimer, similar to a large group of enzymes that, use thiol esters as substrates. Rv0130 was found to contain a highly, conserved R-specific hydratase motif buried deeply between the two, monomers. Our biochemical studies show that the protein is able to hydrate, a short trans-2-enoyl-coenzyme A moiety with a k(cat) of 1.1 x 10(2), sec(-1). The importance of the side chains of D40 and H45 for hydratase, activity is demonstrated by site-directed mutagenesis. In contrast to many, hotdog-folded proteins, a proline residue distorts the central helix of, Rv0130. This distortion allows the creation of a long, curved tunnel, similar to the substrate-binding channels of long-chain eukaryotic, hydratase 2 enzymes.

About this Structure

2C2I is a Single protein structure of sequence from Mycobacterium tuberculosis. Active as Enoyl-CoA hydratase, with EC number 4.2.1.17 Full crystallographic information is available from OCA.

Reference

Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis., Johansson P, Castell A, Jones TA, Backbro K, Protein Sci. 2006 Oct;15(10):2300-9. Epub 2006 Sep 8. PMID:16963641

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