2c4d
From Proteopedia
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2.6A CRYSTAL STRUCTURE OF PSATHYRELLA VELUTINA LECTIN IN COMPLEX WITH N-ACETYLGLUCOSAMINE
Overview
The lectin from the mushroom Psathyrella velutina recognises specifically, N-acetylglucosamine and N-acetylneuraminic acid containing glycans. The, crystal structure of the 401 amino acid residue lectin shows that it, adopts a very regular seven-bladed beta-propeller fold with the N-terminal, region tucked into the central cavity around the pseudo 7-fold axis. In, the complex with N-acetylglucosamine, six monosaccharides are bound in, pockets located between two consecutive propeller blades. Due to the, repeats shown by the sequence the binding sites are very similar. Five, hydrogen bonds between the protein and the sugar hydroxyl and N-acetyl, groups stabilize the complex, together with the hydrophobic interactions, with a conserved tyrosine and histidine. The complex with, N-acetylneuraminic acid shows molecular mimicry with the same hydrogen, bond network, but with different orientations of the carbohydrate ring in, the binding site. The beta-hairpin loops connecting the two inner, beta-strands of each blade are metal binding sites and two to three, calcium ions were located in the structure. The multispecificity and high, multivalency of this mushroom lectin, combined with its similarity to the, extracellular domain of an important class of cell adhesion molecules, integrins, are another example of the outstanding success of, beta-propeller structures as molecular binding machines in nature.
About this Structure
2C4D is a Single protein structure of sequence from Lacrymaria velutina with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Beta-propeller crystal structure of Psathyrella velutina lectin: an integrin-like fungal protein interacting with monosaccharides and calcium., Cioci G, Mitchell EP, Chazalet V, Debray H, Oscarson S, Lahmann M, Gautier C, Breton C, Perez S, Imberty A, J Mol Biol. 2006 Apr 14;357(5):1575-91. Epub 2006 Feb 6. PMID:16497330
Page seeded by OCA on Sun Feb 3 10:31:19 2008
