2c8q
From Proteopedia
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INSULINE(1SEC) AND UV LASER EXCITED FLUORESCENCE
Contents |
Overview
Structural proteomics has promoted the rapid development of automated, protein structure determination using X-ray crystallography. Robotics are, now routinely used along the pipeline from genes to protein structures., However, a bottleneck still remains. At synchrotron beamlines, the success, rate of automated sample alignment along the X-ray beam is limited by, difficulties in visualization of protein crystals, especially when they, are small and embedded in mother liquor. Despite considerable improvement, in optical microscopes, the use of visible light transmitted or reflected, by the sample may result in poor or misleading contrast. Here, the, endogenous fluorescence from aromatic amino acids has been used to, identify even tiny or weakly fluorescent crystals with a high success, rate. The use of a compact laser at 266 nm in combination with, non-fluorescent sample holders provides an efficient solution to collect, high-contrast fluorescence images in a few milliseconds and using standard, camera optics. The best image quality was obtained with direct, illumination through a viewing system coaxial with the UV beam., Crystallographic data suggest that the employed UV exposures do not, generate detectable structural damage.
Disease
Known diseases associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]
About this Structure
2C8Q is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:16510972
Page seeded by OCA on Mon Nov 12 21:12:35 2007
