2ckz

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spinqualitylabelsall models 2ckz, resolution 3.2Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.

Overview

We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a, homology model of the nine subunit core enzyme with a new X-ray structure, of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved, active center for RNA synthesis but a structurally different upstream face, for specific initiation complex assembly during promoter selection. The, Pol III upstream face includes a HRDC domain in subunit C17 that is, translated by 35 A and rotated by 150 degrees compared to its Pol II, counterpart. The HRDC domain is essential in vivo, folds independently in, vitro, and, unlike other HRDC domains, shows no indication of nucleic acid, binding. Thus, the HRDC domain is a functional module that could account, for the role of C17 in Pol III promoter-specific initiation. During, elongation, C17/25 may bind Pol III transcripts emerging from the adjacent, exit pore, because the subcomplex binds to tRNA in vitro.

About this Structure

2CKZ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.

Reference

Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model., Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P, Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233

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