2cv6
From Proteopedia
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Crystal Structure of 8Salpha Globulin, the Major Seed Storage Protein of Mungbean
Overview
The 8S globulins of mung bean [Vigna radiata (L.) Wilczek] are, vicilin-type seed storage globulins which consist of three isoforms:, 8Salpha, 8Salpha' and 8Sbeta. The three isoforms have high sequence, identities with each other (around 90%). The structure of 8Salpha globulin, has been determined for the first time by X-ray crystallographic analysis, and refined at 2.65 A resolution with a final R factor of 19.6% for, 10-2.65 A resolution data. The refined 8Salpha globulin structure, consisted of 366 of the 423 amino-acid residues (one subunit of the, biological trimer). With the exception of several disordered regions, the, overall 8Salpha globulin structure closely resembled those of other seed, storage 7S globulins. The 8Salpha globulin exhibited the highest degree of, sequence identity (68%) and structural similarity (a root-mean-square, deviation of 0.6 A) with soybean beta-conglycinin beta (7S globulin)., Their surface hydrophobicities are also similar to each other, although, their solubilities differ under alkaline conditions at low ionic strength., This difference seems to be a consequence of charge-charge interactions, and not hydrophobic interactions of the surfaces, based on a comparison of, the electrostatic potentials of the molecular surfaces. The thermal, stability of 8Salpha globulin is lower than that of soybean, beta-conglycinin beta. This correlates with the cavity size derived from, the crystal structure, although other structural features also have a, small effect on the protein's thermal stability.
About this Structure
2CV6 is a Protein complex structure of sequences from Vigna radiata. Full crystallographic information is available from OCA.
Reference
Structure of 8Salpha globulin, the major seed storage protein of mung bean., Itoh T, Garcia RN, Adachi M, Maruyama Y, Tecson-Mendoza EM, Mikami B, Utsumi S, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):824-32. Epub 2006, Jun 20. PMID:16790939
Page seeded by OCA on Tue Jan 29 18:48:33 2008
