2cxl

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 2cxl, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

RUN domain of Rap2 interacting protein x, crystallized in I422 space group

Overview

Rap2-interacting protein x (RPIPx) is a homolog of RPIP8, a specific, effector of Rap2 GTPase. The N-terminal region of RPIP8, which contains, the RUN domain, interacts with Rap2. Using cell-free synthesis and NMR, we, determined that the region encompassing residues 83-255 of mouse RPIPx, which is 40-residues larger than the predicted RUN domain (residues, 113-245), is the minimum fragment that forms a correctly folded protein., This fragment, the RPIPx RUN domain, interacted specifically with Rap2B in, vitro in a nucleotide-dependent manner. The crystal structure of the RPIPx, RUN domain was determined at 2.0 A of resolution by the multiwavelength, anomalous dispersion (MAD) method. The RPIPx RUN domain comprises eight, anti-parallel alpha-helices, which form an extensive hydrophobic core, followed by an extended segment. The residues in the core region are, highly conserved, suggesting the conservation of the RUN domain-fold among, the RUN domain-containing proteins. The residues forming a positively, charged surface are conserved between RPIP8 and its homologs, suggesting, that this surface is important for Rap2 binding. In the crystal the, putative Rap2 binding site of the RPIPx RUN domain interacts with the, extended segment in a segment-swapping manner.

About this Structure

2CXL is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the RUN domain of the RAP2-interacting protein x., Kukimoto-Niino M, Takagi T, Akasaka R, Murayama K, Uchikubo-Kamo T, Terada T, Inoue M, Watanabe S, Tanaka A, Hayashizaki Y, Kigawa T, Shirouzu M, Yokoyama S, J Biol Chem. 2006 Oct 20;281(42):31843-53. Epub 2006 Aug 23. PMID:16928684

Page seeded by OCA on Wed Nov 21 09:19:29 2007