2czr
From Proteopedia
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Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA
Overview
TATA-binding protein (TBP)-interacting protein from the hyperthermophilic, archaeon Thermococcus kodakaraensis strain KOD1 (Tk-TIP26) is a possible, transcription regulatory protein in Thermococcales. Here, we report the, crystal structure of Tk-TIP26 determined at 2.3 A resolution with, multiple-wavelength anomalous dispersion (MAD) method. The overall, structure of Tk-TIP26 consists of two domains. The N-terminal domain forms, an alpha/beta structure, in which three alpha-helices enclose the central, beta-sheet. The topology of this domain is similar to that of holliday, junction resolvase Hjc from Pyrococcus furiosus. The C-terminal domain, comprises three alpha-helices, six beta-strands, and two 3(10)-helices. In, the dimer structure of Tk-TIP26, two molecules are related with the, crystallographic twofold axis, and these molecules rigidly interact with, each other via hydrogen bonds. The complex of Tk-TIP26/Tk-TBP is isolated, and analyzed by SDS-PAGE and gel filtration column chromatography, resulting in a stoichiometric ratio of the interaction between Tk-TIP26, and Tk-TBP of 4:2, i.e., two dimer molecules of Tk-TIP26 formed a complex, with one dimeric TBP. The electrostatic surfaces of Tk-TIP26 and TBP from, Pyrocuccus woesei (PwTBP) allowed us to build a model of the Tk-TIP26/TBP, complex, and to propose the inhibition mechanism where two dimer molecules, of Tk-TIP26 bind to a dimeric TBP, preventing its binding to TATA-DNA.
About this Structure
2CZR is a Single protein structure of sequence from Thermococcus kodakarensis with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA., Yamamoto T, Matsuda T, Inoue T, Matsumura H, Morikawa M, Kanaya S, Kai Y, Protein Sci. 2006 Jan;15(1):152-61. Epub 2005 Dec 1. PMID:16322571
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