2dcw
From Proteopedia
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The solution structure of horseshoe crab antimicrobial peptide tachystatin b with the inhibitory cystine-knot motif
Overview
Tachystatin B is an antimicrobial and a chitin-binding peptide isolated, from the Japanese horseshoe crab (Tachypleus tridentatus) consisting of, two isopeptides called tachystatin B1 and B2. We have determined their, solution structures using NMR experiments and distance geometry, calculations. The 20 best converged structures of tachystatin B1 and B2, exhibited root mean square deviations of 0.46 and 0.49 A, respectively, for the backbone atoms in Cys(4)-Arg(40). Both structures have identical, conformations, and they contain a short antiparallel beta-sheet with an, inhibitory cystine-knot (ICK) motif that is distributed widely in the, antagonists for voltage-gated ion channels, although tachystatin B does, not have neurotoxic activity. The structural homology search provided, several peptides with structures similar to that of tachystatin B., However, most of them have the advanced functions such as insecticidal, activity, suggesting that tachystatin B may be a kind of ancestor of, antimicrobial peptide in the molecular evolutionary history. Tachystatin B, also displays a significant structural similarity to tachystatin A, which, is member of the tachystatin family. The structural comparison of both, tachystatins indicated that Tyr(14) and Arg(17) in the long loop between, the first and second strands might be the essential residues for binding, to chitin. Copyright (c) 2007 European Peptide Society and John Wiley &, Sons, Ltd.
About this Structure
2DCW is a Single protein structure of sequence from Tachypleus tridentatus. Full crystallographic information is available from OCA.
Reference
The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an inhibitory cystine-knot motif., Fujitani N, Kouno T, Nakahara T, Takaya K, Osaki T, Kawabata S, Mizuguchi M, Aizawa T, Demura M, Nishimura S, Kawano K, J Pept Sci. 2007 Apr;13(4):269-79. PMID:17394123
Page seeded by OCA on Wed Nov 21 09:32:25 2007
