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2duu

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Revision as of 17:12, 29 January 2008 by OCA (Talk | contribs)
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2duu, resolution 2.9Å

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Crystal Structure of apo-form of NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase from Synechococcus Sp.

Overview

The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate, dehydrogenase (apo-GAPDH) from Synechococcus PCC 7942 is reported. The, crystal structure was solved by molecular replacement and refined to an R, of 21.7% and R(free) of 27.5% at 2.9 angstroms resolution. The structural, features of apo-GAPDH are as follows. The S-loop has an extremely flexible, conformation and the sulfate ion is only taken into the classical P(i), site. A structural comparison with holo-GAPDHs indicated that the S-loop, fixation is essential in the discrimination of NADP and NAD molecules.

About this Structure

2DUU is a Single protein structure of sequence from Synechococcus sp. with as ligand. Active as Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating), with EC number 1.2.1.13 Full crystallographic information is available from OCA.

Reference

Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942., Kitatani T, Nakamura Y, Wada K, Kinoshita T, Tamoi M, Shigeoka S, Tada T, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):727-30. Epub 2006 Jul 29. PMID:16880542

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