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2e0i
From Proteopedia
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Crystal structure of archaeal photolyase from Sulfolobus tokodaii with two FAD molecules: Implication of a novel light-harvesting cofactor
Overview
UV exposure of DNA molecules induces serious DNA lesions. The cyclobutane, pyrimidine dimer (CPD) photolyase repairs CPD-type - lesions by using the, energy of visible light. Two chromophores for different roles have been, found in this enzyme family; one catalyzes the CPD repair reaction and the, other works as an antenna pigment that harvests photon energy. The, catalytic cofactor of all known photolyases is FAD, whereas several, light-harvesting cofactors are found. Currently, 5,10-methenyltetrahydrofolate (MTHF), 8-hydroxy-5-deaza-riboflavin (8-HDF), and FMN are the known light-harvesting cofactors, and some photolyases, lack the chromophore. Three crystal structures of photolyases from, Escherichia coli (Ec-photolyase), Anacystis nidulans (An-photolyase), and, Thermus thermophilus (Tt-photolyase) have been determined; however, no, archaeal photolyase structure is available. A similarity search of, archaeal genomic data indicated the presence of a homologous gene, ST0889, on Sulfolobus tokodaii strain7. An enzymatic assay reveals that ST0889, encodes photolyase from S. tokodaii (St-photolyase). We have determined, the crystal structure of the St-photolyase protein to confirm its, structural features and to investigate the mechanism of the archaeal DNA, repair system with light energy. The crystal structure of the, St-photolyase is superimposed very well on the three known photolyases, including the catalytic cofactor FAD. Surprisingly, another FAD molecule, is found at the position of the light-harvesting cofactor. This second FAD, molecule is well accommodated in the crystal structure, suggesting that, FAD works as a novel light-harvesting cofactor of photolyase. In addition, two of the four CPD recognition residues in the crystal structure of, An-photolyase are not found in St-photolyase, which might utilize a, different mechanism to recognize the CPD from that of An-photolyase.
About this Structure
2E0I is a Single protein structure of sequence from Sulfolobus tokodaii with and as ligands. Active as Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of archaeal photolyase from Sulfolobus tokodaii with two FAD molecules: implication of a novel light-harvesting cofactor., Fujihashi M, Numoto N, Kobayashi Y, Mizushima A, Tsujimura M, Nakamura A, Kawarabayasi Y, Miki K, J Mol Biol. 2007 Jan 26;365(4):903-10. Epub 2006 Oct 7. PMID:17107688
Page seeded by OCA on Tue Jan 29 19:15:11 2008
