2euw
From Proteopedia
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Structure of a Ndt80-DNA complex (MSE mutant mA4T)
Overview
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds, a DNA consensus sequence (the middle sporulation element [MSE]) to, activate gene expression after the successful completion of meiotic, recombination. Here we report the X-ray crystal structures of Ndt80 bound, to ten distinct MSE variants. Comparison of these structures with the, structure of Ndt80 bound to a consensus MSE reveals structural principles, that determine the DNA binding specificity of this transcription factor., The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are, recognized by arginine side chains through a combination of hydrogen, bonding and cation-pi interactions. The 3' AT-rich region is recognized, via minor groove contacts that sterically exclude the N2 atom of GC base, pairs. The conformation of the AT-rich region is fixed by interactions, with the protein that favor recognition of poly(A)-poly(T) versus mixed AT, sequences through an avoidance of major groove steric clashes at 5'-ApT-3', steps.
About this Structure
2EUW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239
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