2f43
From Proteopedia
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Rat liver F1-ATPase
Overview
ATP synthesis from ADP, P(i), and Mg2+ takes place in mitochondria on the, catalytic F1 unit (alpha3beta3gammedeltaepsilon) of the ATP synthase, complex (F0F1), a remarkable nanomachine that interconverts, electrochemical and mechanical energy, producing the high energy terminal, bond of ATP. In currently available structural models of F1, the P-loop, (amino acid residues 156GGAGVGKT163) contributes to substrate binding at, the subunit catalytic sites. Here, we report the first transition, state-like structure of F1 (ADP.V(i).Mg.F1) from rat liver that was, crystallized with the phosphate (P(i)) analog vanadate (VO(3-)4 or V(i))., Compared with earlier "ground state" structures, this new F1 structure, reveals that the active site region has undergone significant remodeling., P-loop residue alanine 158 is located much closer to V(i) than it is to, P(i) in a previous structural model. No significant movements of P-loop, residues of the subunit were observed at its analogous but noncatalytic, sites. Under physiological conditions, such active site remodeling, involving the small hydrophobic alanine residue may promote ATP synthesis, by lowering the local dielectric constant, thus facilitating the, dehydration of ADP and P(i). This new crystallographic study provides, strong support for the catalytic mechanism of ATP synthesis deduced from, earlier biochemical studies of liver F1 conducted in the presence of V(i), (Ko, Y. H., Bianchet, M., Amzel, L. M., and Pedersen, P. L. (1997) J., Biol. Chem. 272, 18875-18881; Ko, Y. H., Hong, S., and Pedersen, P. L., (1999) J. Biol. Chem. 274, 28853-28856).
About this Structure
2F43 is a Protein complex structure of sequences from Rattus norvegicus with VO4, MG, ATP and ADP as ligands. Active as H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 Full crystallographic information is available from OCA.
Reference
Mitochondrial ATP synthase. Crystal structure of the catalytic F1 unit in a vanadate-induced transition-like state and implications for mechanism., Chen C, Saxena AK, Simcoke WN, Garboczi DN, Pedersen PL, Ko YH, J Biol Chem. 2006 May 12;281(19):13777-83. Epub 2006 Mar 10. PMID:16531409
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