This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2f9j
From Proteopedia
|
3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155
Overview
The precise excision of introns from precursor mRNAs (pre-mRNAs) in, eukaryotes is accomplished by the spliceosome, a complex assembly, containing five small nuclear ribonucleoprotein (snRNP) particles. Human, p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown, to associate directly with the pre-mRNA branch adenosine early in, spliceosome assembly and within the fully assembled spliceosome. Here we, report the 2.5-A crystal structure of a complex containing p14 and a, peptide derived from the p14-associated U2 snRNP component SF3b155. p14, contains an RNA recognition motif (RRM), the surface of which is largely, occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide., An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows, that the branch adenosine directly interacts with a conserved aromatic, within a pocket on the surface of the complex. This result, combined with, a comparison of the structure with known RRMs and pseudoRRMs as well as, model-building by using the electron cryomicroscopy structure of a, spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a, noncanonical surface for RNA recognition at the heart of the mammalian, spliceosome.
About this Structure
2F9J is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a core spliceosomal protein interface., Schellenberg MJ, Edwards RA, Ritchie DB, Kent OA, Golas MM, Stark H, Luhrmann R, Glover JN, MacMillan AM, Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1266-71. Epub 2006 Jan 23. PMID:16432215
Page seeded by OCA on Mon Nov 12 22:01:54 2007
