2fip
From Proteopedia
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Phage phi29 transcription regulator p4
Overview
Protein p4 affects the transcriptional switch that divides bacteriophage, phi29 infection in early and late phases. The synthesis of DNA replication, proteins and p4 takes place in the early phase, while structural, morphogenesis, and lysis proteins are synthesized in the late phase., Transcriptional switch by p4 is achieved by activating the late promoter, A3 and repressing the early promoters A2b and A2c. The crystal structure, of p4 alone and in complex with a 41 bp DNA, including the A3 promoter, binding site, helps us to understand how the phage cycle is controlled., Protein p4 has a unique alpha/beta fold that includes a DNA recognition, motif consisting of two N-terminal beta turn substructures, or N-hooks, located at the tips of an elongated protein homodimer. The two N-hooks, enter the major groove of the double helix, establishing base-specific, contacts. A high DNA curvature allows p4 N-hooks to reach two major groove, areas three helical turns apart, like a bow and its string.
About this Structure
2FIP is a Single protein structure of sequence from Vibrio phage f237. Full crystallographic information is available from OCA.
Reference
The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA., Badia D, Camacho A, Perez-Lago L, Escandon C, Salas M, Coll M, Mol Cell. 2006 Apr 7;22(1):73-81. PMID:16600871
Page seeded by OCA on Tue Jan 29 19:37:22 2008
