Inorganic pyrophosphatase
From Proteopedia
Inorganic pyrophosphatase (PPase) catalyzes the breakdown of pyrophosphate (PPi) to two molecules of phosphate (PO4). The reaction releases energy and is irreversible hence it is coupled to biochemical reactions which need to be driven to completion. PPase plays a role in lipid metabolism, calcium absorption, bone formation and DNA synthesis. PPase requires a divalent metal ion like Mg+2 for higher activity. PPi is formed in many biosynthetic reactions which require ATP.
3D structures of inorganic pyrophosphatase
1pyp – yPPase – yeast
1igp, 1faj, 2eip, 1jfd – EcPPase – Escherichia coli
1mjw, 1mjx, 1mjy, 1mjz - EcPPase (mutant)
1b78 - MjPPase – Methanocaldococcus jannaschii
1ude – PPase – Pyrococcus horikoshii
1twl – PPase – Pyrococcus furiosus
1wpm - BsPPase – Bacillus subtilis
1sxv - MtPPase – Mycobacterium tuberculosis
1ygz, 2bqx - HpPPase - Helicobacter pylori
3d53, 3emj - PPase – Rickettsia prowazekii
3d63, 3eiz - BpPPase – Burkholderia pseudomallei
3lo0 - PPase – Ehrlichia chafeensis
3qu5, 3qub, 3quc - BtPPase (mutant) – Bacterioides thetaiotaomicron
3sw5 – PPase – Bartonella henselae
Pyrophosphatase binary complex
1ino – EcPPase + Mn
1ipw, 1obw - EcPPase + Mg
1i40 - EcPPase + Ca
1wgi - yPPase + Mn
1huk - yPPase + Mg
1huj, 2ik1 - yPPase (mutant) + Mg
1qez - PPase + Mg – Sulfolobus acidocaldarius
2mjp - MjPPase + AMPPNP
1k20 - SgPPase + Mn – Streptococcus gordonii
1wpp - SgPPase + Zn
1k23 - BsPPase + Mn
1wpn - BsPPase N terminal + Mn
2bqy - HpPPase + PPi
2uxs - MtPPase + PO4
3ej0 - BpPPase + pyridine derivative
3ej2 - BpPPase + pyrazole derivative
3fq3 - PPase + PO4 – Brucella melitensis
3ld3 - PPase + PO4 – Anaplasma phagocytophilum
3obc - PPase + Zn – Archaeoglobus fulgidus
3quq, 3qu2 - BtPPase + Mg
3qut, 3qu4 - BtPPase (mutant) + Mg
Pyrophosphatase ternary complex
1ypp, 1wgj, 1e6a, 1e9g – yPPase + PO4 + Mn
1m38 - yPPase + PO4 + Co
2ihp - yPPase + PO4 + Mg
117e, 8prk - yPPase (mutant) + PO4 + Mn
2ik0, 2ik4, 2ik6, 2ik7, 2ik9 - yPPase (mutant) + PO4 + Mg
1wcf - MtPPase + PO4 + K
1i6t - EcPPase + PPi + Ca
2au9 - EcPPase + PPi + Mn
2auu - EcPPase + PPi + Mg
2au7 – EcPPase (mutant) + PO4 + Mn
2au8 - EcPPase + PO4 + Mn
2haw - BsPPase + imidodiphosphoric acid + Mg
3qyp, 3qu7 - BtPPase (mutant) + PO4 + Ca
3qu9 - BtPPase (mutant) + tartarate + Mg
3qx7 - BtPPase + PO4 + Mg
3tr4 - PPase + Mg + Mn – Coxiella burnetii
Pyrophosphatase tertiary complex
2au6 - EcPPase + PPi + PO4 + Mn
2iw4 - BsPPase + imidodiphosphoric acid + Fe + Mg + Mn
2enx - PPase + imidodiphosphoric acid + Mg + Mn – Streptococcus agalactiae
3eiy - BpPPase + PPi + Na + K
3gvf - BpPPase + PO4 + Na + K
3qxg - BtPPase + PO4 + Mg + Ca
NADH pyrophosphatase
Proton pyrophosphatase
4a01 – PPase + imidodiphosphoric acid + Mg + K – Vigna radiata
