2ghp

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spinqualitylabelsall models 2ghp, resolution 2.700Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24

Overview

The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein, 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA, base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component, of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not, the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6, base-pairing. The interaction partners of each of the four RRMs of Prp24, and how these interactions direct U4/U6 pairing are not known. Here we, report the crystal structure of the first three RRMs and the solution, structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms, extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy, much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but, leave the beta-sheet of RRM 3 exposed. Previously identified substitutions, in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster, primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM, 2. RNA binding assays and chemical shift mapping indicate that a large, basic patch evident on the surface of RRMs 1 and 2 is part of a high, affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6, RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary, structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through, interaction with an unidentified ligand.

About this Structure

2GHP is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure and interactions of the first three RNA recognition motifs of splicing factor prp24., Bae E, Reiter NJ, Bingman CA, Kwan SS, Lee D, Phillips GN Jr, Butcher SE, Brow DA, J Mol Biol. 2007 Apr 13;367(5):1447-58. Epub 2007 Feb 7. PMID:17320109

Page seeded by OCA on Wed Jan 23 10:38:01 2008