2gi4
From Proteopedia
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Solution Structure of the Low Molecular Weight Protein Tyrosine Phosphatase from Campylobacter jejuni.
Overview
A putative low molecular weight protein tyrosine phosphatase (LMW-PTP) was, identified in the genome sequence of the bacterial pathogen, Campylobacter, jejuni. This novel gene, cj1258, has sequence homology with a distinctive, class of phosphatases widely distributed among prokaryotes and eukaryotes., We report here the solution structure of Cj1258 established by, high-resolution NMR spectroscopy using NOE-derived distance restraints, hydrogen bond data, and torsion angle restraints. The three-dimensional, structure consists of a central four-stranded parallel beta-sheet flanked, by five alpha-helices, revealing an overall structural topology similar to, those of the eukaryotic LMW-PTPs, such as human HCPTP-A, bovine BPTP, and, Saccharomyces cerevisiae LTP1, and to those of the bacterial LMW-PTPs, MPtpA from Mycobacterium tuberculosis and YwlE from Bacillus subtilis. The, active site of the enzyme is flexible in solution and readily adapts to, the binding of ligands, such as the phosphate ion. An NMR-based screen was, carried out against a number of potential inhibitors and activators, including phosphonomethylphenylalanine, derivatives of the cinnamic acid, 2-hydroxy-5-nitrobenzaldehyde, cinnamaldehyde, adenine, and hypoxanthine., Despite its bacterial origin, both the three-dimensional structure and, ligand-binding properties of Cj1258 suggest that this novel phosphatase, may have functional roles close to those of eukaryotic and mammalian, tyrosine phosphatases. The three-dimensional structure along with mapping, of small-molecule binding will be discussed in the context of developing, high-affinity inhibitors of this novel LMW-PTP.
About this Structure
2GI4 is a Single protein structure of sequence from Campylobacter jejuni. Active as Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Full crystallographic information is available from OCA.
Reference
Three-dimensional structure and ligand interactions of the low molecular weight protein tyrosine phosphatase from Campylobacter jejuni., Tolkatchev D, Shaykhutdinov R, Xu P, Plamondon J, Watson DC, Young NM, Ni F, Protein Sci. 2006 Oct;15(10):2381-94. PMID:17008719
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