2grw
From Proteopedia
|
Solution structure of the poliovirus 3'-UTR Y-stem
Overview
The previously described NMR structure of a 5'-CU-3'/5'-UU-3' motif, which, is highly conserved within the 3'-UTR Y-stem of poliovirus-like, enteroviruses, revealed striking regularities of the local helix geometry, thus retaining the pseudo-twofold symmetry of the RNA helix. A mutant, virus with both pyrimidine base pairs changed into Watson-Crick replicated, as wild type, indicating the functional importance of this symmetry, relation in viral RNA replication. Here we investigated the effect of, changing only one of the two pyrimidine base pairs to Watson-Crick. We, determined the NMR structures of two Y-stem variants: one containing the, 5'-CU-3'/5'-AU-3' motif, which has been found in wild-type virus isolates, as well, and the other containing a 5'-CU-3'/5'-UG-3' motif, which is not, present in any enterovirus sequenced to date. Both structures show single, pyrimidine mismatches with intercalated bases. In the 5'-CU-3'/5'-AU-3', motif a C-U Watson-Crick-type base pair is formed that retains the, pseudo-twofold symmetry, while in the 5'-CU-3'/5'-UG-3' motif a single, asymmetric U-U mismatch breaks the twofold symmetry. Surprisingly, for the, nonnatural variant no effect of the single base-pair replacement was, observed on polioviral RNA replication using an in vitro replicon assay.
About this Structure
2GRW is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Breaking pseudo-twofold symmetry in the poliovirus 3'-UTR Y-stem by restoring Watson-Crick base pairs., Zoll J, Tessari M, Van Kuppeveld FJ, Melchers WJ, Heus HA, RNA. 2007 May;13(5):781-92. PMID:17449731
Page seeded by OCA on Wed Jan 23 15:18:19 2008
