2h0x
From Proteopedia
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Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
Overview
The glmS ribozyme is the only natural catalytic RNA known to require a, small-molecule activator for catalysis. This catalytic RNA functions as a, riboswitch, with activator-dependent RNA cleavage regulating glmS, messenger RNA expression. We report crystal structures of the glmS, ribozyme in precleavage states that are unliganded or bound to the, competitive inhibitor glucose-6-phosphate and in the postcleavage state., All structures superimpose closely, revealing a remarkably rigid RNA that, contains a preformed active and coenzyme-binding site. Unlike other, riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of, the glmS ribozyme-bound coenzyme performs general acid-base and, electrostatic catalysis.
About this Structure
2H0X is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543
Page seeded by OCA on Tue Jan 29 20:11:54 2008
Categories: Single protein | Amare, A.R.Ferre-D. | Klein, D.J. | MG | Helix | Pseudoknot | Ribozyme | Rna
