2h1y

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spinqualitylabelsall models 2h1y, resolution 2.500Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of malonyl-CoA:Acyl carrier protein transacylase (MCAT) from Helicobacter pylori

Overview

Malonyl-CoA: acyl carrier protein transacylase (MCAT) is a critical enzyme, responsible for the transfer of the malonyl moiety to holo-acyl carrier, protein (ACP) forming the malonyl-ACP intermediates in the initiation step, of type II fatty acid synthesis (FAS II) in bacteria. MCAT has been, considered as an attractive drug target in the discovery of antibacterial, agents. In this study, the crystal structure of MCAT from Helicobacter, pylori (Hp) at 2.5 A resolution is reported, and the interaction of HpMCAT, with HpACP is extensively investigated by using computational docking, GST-pull-down, and surface plasmon resonance (SPR) technology-based, assays. The crystal structure results reveal that HpMCAT has a compact, folding composed of a large subdomain with a similar core as in alpha/beta, hydrolases, and a similar ferredoxin-like small subdomain as in, acylphosphatases. The docking result suggests two positively charged areas, near the entrance of the active site of HpMCAT as the ACP-binding region., Binding assay research shows that HpMCAT demonstrates a moderately binding, ability against HpACP. The solved 3D structure of HpMCAT is expected to, supply useful information for the structure-based discovery of novel, inhibitors against MCAT, and the quantitative study of HpMCAT interaction, with HpACP is hoped to give helpful hints in the understanding of the, detailed catalytic mechanisms for HpMCAT.

About this Structure

2H1Y is a Single protein structure of sequence from Helicobacter pylori. Active as [Acyl-carrier-protein_S-malonyltransferase [Acyl-carrier-protein] S-malonyltransferase], with EC number 2.3.1.39 Full crystallographic information is available from OCA.

Reference

Malonyl-CoA: acyl carrier protein transacylase from Helicobacter pylori: Crystal structure and its interaction with acyl carrier protein., Zhang L, Liu W, Xiao J, Hu T, Chen J, Chen K, Jiang H, Shen X, Protein Sci. 2007 Jun;16(6):1184-92. PMID:17525466[[Category: [Acyl-carrier-protein] S-malonyltransferase]]

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