2hki
From Proteopedia
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Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform
Overview
Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate, dehydrogenase) from Spinacia oleracea were obtained using sitting-drop, vapor diffusion. Despite the very low concentration of GADPH in the, solutions, two crystalline forms were obtained, one of which was the, previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space, group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A., Diffraction data were measured from both native and derivatives, yielding, structures at a resolution limit of 3.0 A. Differences at the, NAD(+)/NADP(+)-binding site seen in these structures compared with the, previously reported structure with bound coenzyme suggest that, conformational changes associated with pyridine-nucleotide binding may, play a role in the regulation of this enzyme.
About this Structure
2HKI is a Single protein structure of sequence from Spinacia oleracea with SO4 as ligand. Active as Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating), with EC number 1.2.1.13 Full crystallographic information is available from OCA.
Reference
Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:17077485
Page seeded by OCA on Wed Nov 21 11:49:18 2007
