2hn8
From Proteopedia
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Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein
Overview
Recently, a novel 87-amino acid influenza A virus protein with, proapoptotic properties, PB1-F2, has been reported that originates from an, alternative reading frame in the PB1 polymerase gene and is encoded in, most known human influenza A virus isolates. Here we characterize the, molecular structure of a biologically active synthetic version of the, protein (sPB1-F2). Western blot analysis, chemical cross-linking, and NMR, spectroscopy afforded direct evidence of the inherent tendency of sPB1-F2, to undergo oligomerization mediated by two distinct domains located in the, N and C termini, respectively. CD and (1)H NMR spectroscopic analyses, indicate that the stability of structured regions in the molecule clearly, depends upon the hydrophobicity of the solvent. In aqueous solutions, the, behavior of sPB1-F2 is typical of a largely random coil peptide that, however, adopts alpha-helical structure upon the addition of membrane, mimetics. (1)H NMR analysis of three overlapping peptides afforded, for, the first time, direct experimental evidence of the presence of a, C-terminal region with strong alpha-helical propensity comprising amino, acid residues Ile(55)-Lys(85) connected via an essentially random coil, structure to a much weaker helix-like region, located in the N terminus, between residues Trp(9) and Lys(20). The C-terminal helix is not a true, amphipathic helix and is more compact than previously predicted. It, corresponds to a positively charged region previously shown to include the, mitochondrial targeting sequence of PB1-F2. The consequences of the strong, oligomerization and helical propensities of the molecule are discussed and, used to formulate a hypothetical model of its interaction with the, mitochondrial membrane.
About this Structure
2HN8 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structural characterization and oligomerization of PB1-F2, a proapoptotic influenza A virus protein., Bruns K, Studtrucker N, Sharma A, Fossen T, Mitzner D, Eissmann A, Tessmer U, Roder R, Henklein P, Wray V, Schubert U, J Biol Chem. 2007 Jan 5;282(1):353-63. Epub 2006 Oct 19. PMID:17052982
Page seeded by OCA on Wed Nov 21 11:51:32 2007
