2knt
From Proteopedia
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THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5
Contents |
Overview
The recombinant Kunitz-type domain (C5) of human collagen alpha3(VI) chain, was previously described at 1.6 A resolution at room temperature. By, changing the crystallization conditions and using synchrotron radiation, we are able to record diffraction data to 1.2 A resolution for crystals of, the same space group at 291 K. The protein-water-ion model has been, refined anisotropically against these new data using the program SHELXL93;, the results converged to an R factor of 15.0%, with all data between 7 and, 1.2 A. The final electron-density map reveals a clear chain tracing with a, few disordered residues and five residues out of 58 that present alternate, conformations. The Cys14-Cys38 bond presents the less frequently observed, left-hand conformation (chi1 = -60 degrees). The solvent molecules and a, phosphate ion are well ordered with an average B of 38 A2. The, high-resolution structure reveals the N and C termini which were missing, from the 1.6 A structure.
Disease
Known diseases associated with this structure: Bethlem myopathy OMIM:[120250], Ullrich congenital muscular dystrophy OMIM:[120250]
About this Structure
2KNT is a Single protein structure of sequence from Homo sapiens with PO4 as ligand. Full crystallographic information is available from OCA.
Reference
1.2 A refinement of the Kunitz-type domain from the alpha3 chain of human type VI collagen., Merigeau K, Arnoux B, Perahia D, Norris K, Norris F, Ducruix A, Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):306-12. PMID:9761897
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