2o42
From Proteopedia
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Crystal Structure of M11L, Bcl-2 homolog from myxoma virus
Overview
Apoptosis of virally infected cells is an innate host mechanism used to, prevent viral spread. However, viruses have evolved a number of proteins, that function to modulate the apoptotic cascades and thereby favor, productive viral replication. One such antiapoptotic protein, myxoma virus, M11L, has been shown to inhibit mitochondrial-dependent apoptosis by, binding to and blocking the two executioner proteins Bak and Bax. Since, M11L has no obvious sequence homology with Bcl-2 or Bcl-x(L), the normal, cellular inhibitors for Bak and Bax, and the structure of M11L has not, been solved, the mode of binding to Bak and Bax is not known. In order to, understand how M11L functions, the crystal structure of M11L was solved to, 2.91 A. Despite the lack of sequence similarity, M11L is a structural, homolog of Bcl-2. Studies using a peptide derived from Bak indicate that, M11L binds to Bak with a similar affinity (4.9 +/- 0.3 microM) to the, published binding affinities of Bcl-2 and Bcl-x(L) to the same peptide, (12.7 microM and 0.5 microM, respectively), indicating that M11L inhibits, apoptosis by mimicking and competing with host proteins for the binding of, Bak and Bax. The structure provides important insight into how myxoma, virus and other poxviruses facilitate viral dissemination by inhibiting, mitochondrial dependent apoptosis.
About this Structure
2O42 is a Single protein structure of sequence from Myxoma virus. Full crystallographic information is available from OCA.
Reference
Structure of M11L: A myxoma virus structural homolog of the apoptosis inhibitor, Bcl-2., Douglas AE, Corbett KD, Berger JM, McFadden G, Handel TM, Protein Sci. 2007 Apr;16(4):695-703. PMID:17384234
Page seeded by OCA on Wed Jan 23 12:03:06 2008
