User:Alice Harmon/Sandbox 4

From Proteopedia

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Arabidopsis SnRK2.6 (OST1)

spinqualitylabelsall models 3uc4 - apo SnRK2.6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SNRK2 box is a C-terminal motif that is common to all SNRK2s. It forms a helix that parallels subdomain III.

Activation loop (with gap) in blue
Catalytic loop in orchid
C-helix in yellow
K in chartreuse
Snrk2 box (unique to Snrk's) in turquoise.
C-terminal acidic domain missing.

spinqualitylabelsall models 3ujg - SnRK2.6 in complex with HAB1 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The SnRK2.6–HAB1 complex was constructed as a fusion protein with a H6-tag (MAHHHHHHA) at the N-terminus of SnRK2.6 (residues 11–362) fused to HAB1(172–511) with a GSGSAGSAAGS linker.

D296A and E297A surface entropy reduction mutation sites

ABA box is acidic sequence in C-terminus that is common to SNRK2s that are regulated by ABA. This sequence binds to PP2C in biochemical assays, but is not seen in the crystal structure.

kinase activation loop in blue
phosphatase W385 in brown

unused:

SNRK2 structures

3uc3 Arabidopsis thaliana SNRK2.3 + Co²⁺
3zut AtSNRK2.6 (D160A mutant)+ ANP
3zuu AtSNRK2.6 (D160A, S175D mutant) + gold
3uc4 apoAtSNRK2.6 (D59A, E60A mutant)
3udb apoAtSNRK2.6 (C131A, C157A, C159A, S7A, s29A, s43A, S166A, T175A)

complex with a protein phosphatase 2C
3ujg AtSNRK2.6 (D296A) + HAB1 + Mg²⁺

PP2C structures

1a6q – hPP2C
2iq1 – hPP2C κ
2p8e – hPP2C β
2cm1 – PP2C + Mn – Micobacterium tuberculosis
3d8k – PP2C – Toxoplasma gondii
3jrq, 3kdj, 3nmn – AtPP2C + Pyl1 – Arabidopsis thaliana
3nmt, 3kb3, 3nmv, 3ujl – AtPP2C + Pyl2
4ds8 – AtPP2C + Pyl3 + Mn
3rt0 – AtPP2C (mutant) + Pyl10
3qn1, 3zvu – AtPP2C + Pyr1
3ujg – AtPP2C + SNRK2
3ujk – AtPP2C