2ocj

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spinqualitylabelsall models 2ocj, resolution 2.050Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Human p53 core domain in the absence of DNA

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The tumor suppressor protein p53 plays a key role in cell-cycle regulation, by triggering DNA repair, cell-cycle arrest and apoptosis when the, appropriate signal is received. p53 has the classic architecture of a, transcription factor, with an amino-terminal transactivation domain, a, core DNA-binding domain and carboxy-terminal tetramerization and, regulatory domains. The crystal structure of the p53 core domain, which, includes the amino acids from residue 96 to residue 289, has been, determined in the absence of DNA to a resolution of 2.05 A. Crystals grew, in a new monoclinic space group (P2(1)), with unit-cell parameters a =, 68.91, b = 69.36, c = 84.18 A, beta = 90.11 degrees . The structure was, solved by molecular replacement and has been refined to a final R factor, of 20.9% (R(free) = 24.6%). The final model contains four molecules in the, asymmetric unit with four zinc ions and 389 water molecules. The, non-crystallographic tetramers display different protein contacts from, those in other p53 crystals, giving rise to the question of how p53, arranges as a tetramer when it binds its target DNA.

Disease

Known diseases associated with this structure: Adrenal cortical carcinoma OMIM:[191170], Breast cancer OMIM:[191170], Colorectal cancer OMIM:[191170], Hepatocellular carcinoma OMIM:[191170], Histiocytoma OMIM:[191170], Li-Fraumeni syndrome OMIM:[191170], Multiple malignancy syndrome OMIM:[191170], Nasopharyngeal carcinoma OMIM:[191170], Osteosarcoma OMIM:[191170], Pancreatic cancer OMIM:[191170], Thyroid carcinoma OMIM:[191170]

About this Structure

2OCJ is a Single protein structure of sequence from Homo sapiens with ZN as ligand. Full crystallographic information is available from OCA.

Reference

Structure of the human p53 core domain in the absence of DNA., Wang Y, Rosengarth A, Luecke H, Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):276-81. Epub 2007, Feb 21. PMID:17327663

Page seeded by OCA on Mon Nov 12 23:09:12 2007