2owo
From Proteopedia
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Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate
Overview
NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria and, essential for growth. Their distinctive substrate specificity and domain, organization vis-a-vis human ATP-dependent ligases make them outstanding, targets for anti-infective drug discovery. We report here the 2.3 A, crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the, basis for nick recognition. LigA envelops the DNA within a protein clamp., Large protein domain movements and remodeling of the active site, orchestrate progression through the three chemical steps of the ligation, reaction. The structure inspires a strategy for inhibitor design.
About this Structure
2OWO is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as DNA ligase (NAD(+)), with EC number 6.5.1.2 Full crystallographic information is available from OCA.
Reference
Last Stop on the Road to Repair: Structure of E. coli DNA Ligase Bound to Nicked DNA-Adenylate., Nandakumar J, Nair PA, Shuman S, Mol Cell. 2007 Apr 27;26(2):257-71. PMID:17466627
Page seeded by OCA on Wed Jan 23 15:17:07 2008
Categories: DNA ligase (NAD(+)) | Escherichia coli | Single protein | Nair, P.A. | Nandakumar, J. | Shuman, S. | AMP | SO4 | ZN | Dna | Ligase | Protein/dna complex
